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We need to get plate counts between 30 and 300, and we think that the autocad 2007 system requirements concentration in his blood might be as high as 500 per mL, but it could be much lower.
Let s say I guesstimate that I have about 42 million CFUs per mL - I might want to start with a first dilution of about 1/1000, and plate that.
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MathBench Measurement Serial Dilution: Counting Small Round Things This module is currently under revision In this module, we re going to consider some practical implications of counting bacteria.Blaaah, still too much.That way, each liter of soup will have only 90,000 peas instead of 9 million.That s pretty good.In order to get a 0,000 dilution, you need to put 1 mL into _ mLs of water.This is where dilution saves the day.And, if you roll over the image, you will see the plate counts I expect if my original guess 42 million was approximately correct.Since we cleverly labeled each plate with its overall dilution factor, we also know how to scale back up to get the original concentration.I think I have the answer: 0,000.We ll talk about a specific method serial dilution, plating, counting, and scaling up, but I want to make it clear at the outset that the problem of counting huge numbers of things is not at all unique to counting bacteria.It doesn t really matter how much stock you start with, the important thing is to take out the right fraction of it a tenth, a hundredth and then add enough water to get back to the initial volume.That would be pretty difficult with a pipette.This rule of thumb has the undeniable advantage of being easy to use.Let s say you need to get 1/10,000th of a mL in order to count the bacteria.If you want a printer-friendly version of this module, you can find it here in a Microsoft Word document.Also, notice in this problem I didn t tell you how much the small bag weighed, only that it contained 1/11 of the amount of a large bag.Take 1 mL of that and put it in 49 mLs of water.Use the scratchpad to check your plans, and if you get completely lost, click the.500,000 bacteria: the diluted sample will contain about 50 CFUs, which is both easy to count and valid for scaling.
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